fa Morphometry Morphometry Original Original <strong>Purpose:</strong> The purpose of this study is to evaluate the effect of Di-(2-ethylhexyl) phthalate (DEHP) on testicular spermatid number per gram testis (TSN), daily sperm production (DSP), count, motility, viability, morpholo<u>gy</u>, and chromatine quality of epididymal sperm. <strong>Materials and Methods</strong>: The protocol for DEHP administration was that adult male NMRI mice (the <u>age </u>group of 4 weeks) received 29 DEHP/100ul corn oil/kg, and vehicle group received 100ul corn oil/kg by gavage for 14 days. The control group did not receive DEHP. All the samples were assessed according to World Health Organization (WHO) criteria. Sperm morpholo<u>gy </u>was assessed using papanicula staining. Sperm chromatine quality was assessed usin<u>g </u>aniline-blue staining. <strong>Results</strong>: Administration of DEHP induced significantly reduction of TSN, DSP and epididymal sperm count (p<0.05). The percentage of motility, viability, normal morphology, and chromosomal quality were significantly low in comparison with control group (p<0.05). <strong>Conclusion</strong>: These results demonstrated that DEHP administration has toxic effects on TSN, DSP, epididymal sperm parameter and finally on male reproductive system.&nbsp; Di-(2-ethylhexyl) phthalate, Daily sperm production, Sperm parameters, Toxic effects 0 0 http://anatomyjournal.ir/browse.php?a_code=A-10-304-250&slc_lang=fa&sid=1 Zohre Zare 10031947532846005918 10031947532846005918 Yes Hossein Eimani 10031947532846005919 10031947532846005919 No Moslem Mohammadi 10031947532846005920 10031947532846005920 No Mahmoud Mofid 10031947532846005921 10031947532846005921 No Hossein Dashtnavard 10031947532846005922 10031947532846005922 No