fa Morphometry Morphometry Original Original <strong>Purpose</strong>: The aim of this study was to evaluate the effect of NT-3 on the decrease of the differentiation time of bulge stem cells of rat hair follicle from neuron like cells.<strong>Materials and Methods</strong>: The bulge region of the rat whisker was isolated from and cultured in DMEM/F12 supplemented with epidermal growth factor (EGF) in 3 groups for 7, 8 and 9 days .Then 10 ng/ml NT-3 was added to each group for 3 days. Finally, cell differentiation was assessed by immunocytochemistry using &beta;Ⅲ-tubulin antibody and the result was compared with control groups.<strong>Results</strong>: According to our results hair follicle bulge stem cells expressed CD34 and Nestin in 7-9 and 10-12 first days after cultivation respectively. By using NT-3 duration differentiation process the cells showed positive reaction to &beta;Ⅲ-tubulin antibody.<strong>Conclusion</strong>: The results indicate that NT-3 can affect on differentiation speed even in less than 10 days Bulge of Hair Follicle, Cd34, Nestin, Nt-3, ΒⅢ-Tubulin 0 0 http://anatomyjournal.ir/browse.php?a_code=A-10-304-238&slc_lang=fa&sid=1 Fatemeh Moghani Ghoroghi 10031947532846006334 10031947532846006334 No Maliheh Nobakht 10031947532846006335 10031947532846006335 Yes Banfsheh Esmaeilzade 10031947532846006336 10031947532846006336 No Mehrdad Bakhtiyari 10031947532846006337 10031947532846006337 No Leila Beygom Hejazian 10031947532846006338 10031947532846006338 No