fa Morphometry Morphometry Original Original <strong>Purpose</strong>:&nbsp;To improve the development of frozen mouse embryos by adding fibroblast growth factors (FGF) and hepatocytes (HGF) to the culture medium.<strong>&nbsp;Materials and Methods</strong>: Two-cell mouse embryos obtained from the fallopian tube and frozen by cryotopic method were treated in T6 medium with a dose of 20 ng / ml FGF and 20 ng / ml HGF. The results of their culture were compared with control groups and the rate of embryonic development was evaluated.<strong>Results</strong>:&nbsp;The results showed that the rate of cleavage and development of vitrified and non-vitrified mouse embryos in the culture medium by adding growth factors was significantly increased compared to the control group (p <0.01).&nbsp;There was also a significant difference in the rate of morula development between vitrified and non-vitrified embryos treated with growth factors (p <0.05), but this difference was not observed in the rate of blastocyst formation (p> 0.05).&nbsp;<strong>Conclusion:</strong> The results of this study show that the addition of growth factors in the culture medium improves the pre-implantation development of mouse embryos after vitrification. Growth Factors, Pre-implantation Development, Vitrification 0 0 http://anatomyjournal.ir/browse.php?a_code=A-10-304-237&slc_lang=fa&sid=1 Fatemeh Ghasemian 10031947532846006228 10031947532846006228 No Mahnaz Azarnia 10031947532846006229 10031947532846006229 No Masoumeh Faghani Langroudi 10031947532846006230 10031947532846006230 No Mohammad Hadi Bahadori 10031947532846006231 10031947532846006231 Yes