fa Morphometry Morphometry Original Original <strong>Purpose</strong>: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it.<br> <strong>Materials and methods</strong>: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restriction enzyme .Then frame mutation was prepared and sequenced.<br> <strong>Results</strong>: glucocerebrosidase cDNA was synthesized and amplified by PCR. Then cloned in pUCBM21 and confirmed using restriction analysis. An in frame mutation was done and confirmed by sequencing.<br> <strong>Conclusion</strong>: The successful sequenced Gba cDNA compared with other researchers results. Glucocerebrosidase, Cloning, Pucbm21 in frame mutation 185 190 http://anatomyjournal.ir/browse.php?a_code=A-10-304-109&slc_lang=fa&sid=1 Homayoun Naderian 10031947532846006193 10031947532846006193 Yes Anatomy Department, Medical Facalty, Kashan Medical Sciences University, Kashan, Iran. Bahram Kazemi 10031947532846006194 10031947532846006194 No Ahmad Hoseini 10031947532846006195 10031947532846006195 No Yoosef Sadeghi 10031947532846006196 10031947532846006196 No Antovan De Vries 10031947532846006197 10031947532846006197 No