Anatomical Sciences Journal
مجله علوم تشریح
ASJ
Basic Sciences
http://anatomyjournal.ir
1
admin
17286158
17286158
en
jalali
1394
2
1
gregorian
2015
5
1
12
2
online
1
fulltext
en
The Effect of Mesenchymal Stem Cells and Aqueous Extract of Elaeagnus Angustifolia on the Mechanical Properties of Articular Cartilage in an Experimental Model of Rat Osteoarthritis
Editorial
Editorial
<p dir="ltr" style="text-align: justify"><strong>Introduction:</strong> Although, the effect of direct intra-articular injection of bone marrow stem cells (BMSCs) on the repair of articular cartilage and the effect of Elaeagnus angustifolia extract on pain relief in patients with osteoarthritis have been investigated, no studies has been conducted to compare the effects of these two therapeutic methods on the mechanical properties of articular cartilage. In the present stuy, the effect of these two methods on the mechanical strength of knee articular cartilage in a model of rat osteoarthritis has been studied.<br>
<strong>Methods:</strong> In the present research, 48 mature, male Wistar rats were used. Animals were randomly divided into 6 groups of 8 as follows: control group (healthy animals), saline with mono-iodoacetate (MIA), MIA with Elaeagnus angustifolia extract, MIA with BMSCs, and MIA with a combination of Elaeagnus angustifolia extract and BMSCs. Osteoarthritis was induced by injection of 50 &muL solution of MIA in rats of groups 3 to 6. About 500 mg/kg Elaeagnus angustifolia extract was injected intraperitoneally daily for 4 weeks and nonautologous mesenchymal stem cells were injected into the knee joint on the 14th day. Stress-relaxation test was conducted applying 0.1 mm displacement at the rate of 5 mm/min for 1000 seconds. Then, the maximum initial force, instantaneous stiffness,equilibrium force, and equilibrium stiffness were calculated.</p>
<p dir="ltr" style="text-align: justify"><span style="line-height: 1.6em"><strong>Results:</strong> Induction of osteoarthritis model decreased instantaneous stiffness, maximum initial force, </span><span style="line-height: 1.6em">and equilibrium stiffness as compared to the healthy group (P=0.05). Using Elaeagnus angustifolia </span><span style="line-height: 1.6em">extract and bone marrow stem cells increased instantaneous stiffness and equilibrium stiffness </span><span style="line-height: 1.6em">compared to MIA group, although this increase was statistically significant only in the BMSCs </span><span style="line-height: 1.6em">group (P=0.04 and P=0.026, respectively). In the BMSCs group, maximum initial force also </span><span style="line-height: 1.6em">significantly increased compared to MIA group (P=0.04).</span></p>
<p dir="ltr" style="text-align: justify"><strong>Conclusion:</strong> Apparently direct injection of BMSCs into the knee joint with osteoarthritis is more effective in increasing mechanical strength of the cartilage and improving the performance of the weight-bearing joint compared to using Elaeagnus angustifolia extract.</p>
Osteoarthritis, Mesenchymal stem cells, Elaeagnus angustifolia, Mechanical strength of cartilage
68
74
http://anatomyjournal.ir/browse.php?a_code=A-10-101-1&slc_lang=en&sid=1
Mahmoud
Mofid
mofidmmail@yahoo.com
10031947532846001026
10031947532846001026
No
Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Seyed Homayoon
Sadraie
h_sadraie@yahoo.com
10031947532846001027
10031947532846001027
Yes
Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Hossein
Imani
Eimanih@yahoo.com
10031947532846001028
10031947532846001028
No
Department of Anatomical Sciences, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Giti
Torkaman
torkamang@yahoo.com
10031947532846001029
10031947532846001029
No
Department of Physiotherapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Gholamreza
Kaka
gh_kaka@yahoo.com
10031947532846001030
10031947532846001030
No
Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Mohammad Reza
Naghii
naghiimr@yahoo.com
10031947532846001031
10031947532846001031
No
Department of Nutrition, Health School, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Gholamhossein
Alishiri
ghalishiri@yahoo.com
10031947532846001032
10031947532846001032
No
Baqiyatallah University of Medical Sciences, Tehran, Iran
Mohammad Hossein
Asadi
asadiamh@yahoo.com
10031947532846001033
10031947532846001033
No
Department of Rheumatology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.