en Original Original <div dir="ltr" style="text-align: justify"><strong>Introduction:</strong> Multiple sclerosis (MS) is a disease of the immune system: it attacks the myelin around the axons and leaves them exposed. Destruction of myelin weakens the electrical conduction of ions and thus leads to a lack of communication in the nervous system. </div> <div dir="ltr" style="text-align: justify"><strong>Methods:</strong> In the present study, we constructed recombinant plasmid and then transformed to E. coli cell. The colonies containing plasmid were selected by Colony PCR. Enzyme digestion and sequencing were utilized to approve the accuracy of the extracted plasmid of these clones. Recombinant plasmid transfect in to mesanchymal stem cells.<br> <strong>Results:</strong> Plasmid was verified correctly. After transfection, the transcription of MOG gene and the expression of MOG protein were proved by RT-PCR, western blotting and Elisa.<br> <strong>Conclusion: </strong>Plasmid was constructed correctly and mesenchyme stem cells were successfully transfected by transfection and protein can be expressed well, setting a proper foundation for the future studies on the transplantation of gene modified mesanchymal stem cells in order to promote Multiple sclerosis.</div> MOG, Multiple sclerosis, Gene therapy 3 8 http://anatomyjournal.ir/browse.php?a_code=A-10-87-1&slc_lang=en&sid=1 Mohammad Hassan Karimfar 1003194753284600902 1003194753284600902 No Organization Mohsen Noorozian 1003194753284600903 1003194753284600903 No Organization Reza Mastery Farahani 1003194753284600904 1003194753284600904 No Organization Maryam Sadat Khoramgah 1003194753284600905 1003194753284600905 No Organization Hadi Azimi 1003194753284600906 1003194753284600906 No Organization Ali Asghar Keramatinia 1003194753284600907 1003194753284600907 No Organization Navid Ahmadi Rozbahani 1003194753284600908 1003194753284600908 No Organization Ayad Bahadori Monfared 1003194753284600909 1003194753284600909 No Organization Hojjat-Allah Abbaszadeh hoomanabs@gmail.com 1003194753284600910 1003194753284600910 Yes Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran.