AU - Taheri, Hossein AU - Salehi, Mohammad AU - Eslami Arshaghi, Tarlan AU - Mosahebi, Majid TI - Studying of the Expression of BAX and BCL-2 Genes in Men with Sperm DNA Fragmentation Based on DFI and Comet Assay PT - JOURNAL ARTICLE TA - ASJ JN - ASJ VO - 12 VI - 1 IP - 1 4099 - http://anatomyjournal.ir/article-1-81-en.html 4100 - http://anatomyjournal.ir/article-1-81-en.pdf SO - ASJ 1 AB  - Introduction: The amount of expression of BAX and BCL-2 genes in infertile men’s sperm as well as its association with sperm parameters and DNA fragmentation index is an issue which has not been studied yet. In this research, it is assumed that up-regulation of BAX and downregulation of BCL-2 are directly associated with sperm DNA fragmentation. Methods: After obtaining semen samples from the patients by using gradient centrifugation method, the semen samples were centrifuged using gradient method in order to obtain pure sperm. Sperm is divided into three parts based on which flow cytometry, Real Time-PCR and Comet Assay techniques were conducted. After extracting RNA and producing cDNA, the amount of expression of BAX and BCL-2 genes was measured using Real Time-PCR. The amount of sperm DNA fragmentation was measured using flow cytometry and Comet Assay techniques. Based on the amount of DNA fragmentation index (DFI), samples were divided into the two groups of control (DFI CP - IRAN IN - Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences and Health Services, Tehran, Iran. LG - eng PB - ASJ PG - 29 PT - Original YR - 2015