Volume 3, Issue 1 (Spring 2005)
ASJ 2005, 3(1): 11-18 |
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1- Department of stem cells, Royan Institute, Tehran, Iran.
Abstract: (894 Views)
Purpose: Differentiation of Mouse embryonicstem cells into Hematopoietic cells.
Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed by RT-PCR and colony forming assay.
Results: RT-PCR results showed the expression of two chain of mouse hemoglobin in differentiated cell. Also, colony forming assay confirmed results of RT-PCR. Colony of hematopoietic cells and benzidine positive cells that are representative of erythroid colonies were seen in semisolid media.
Conclusion: The results of this study showed that cultivation of EBs in suspension medium with hematopoietic inducer cytokines is effective method for differentiation of embryonic stem cells into hematopoietic cells.
Materials and Methods: In this study, we used EB formation system for Hematopoietic differentiation of mouse embryonic stem cell (Royan B1) in suspension culture. EBs cultured in medium with Hematopoietic inducer cytokines (SCF, TPO, GMCSF, IL3, Flt3 and EPO) .presence of hematopoietic differentiated cell assessed by RT-PCR and colony forming assay.
Results: RT-PCR results showed the expression of two chain of mouse hemoglobin in differentiated cell. Also, colony forming assay confirmed results of RT-PCR. Colony of hematopoietic cells and benzidine positive cells that are representative of erythroid colonies were seen in semisolid media.
Conclusion: The results of this study showed that cultivation of EBs in suspension medium with hematopoietic inducer cytokines is effective method for differentiation of embryonic stem cells into hematopoietic cells.
Type of Study: Original |
Subject:
Morphometry
Received: 2021/12/26 | Accepted: 2005/04/21 | Published: 2005/04/21
Received: 2021/12/26 | Accepted: 2005/04/21 | Published: 2005/04/21
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