Volume 1, Issue 1 (Winter 2003)                   ASJ 2003, 1(1): 1-16 | Back to browse issues page

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Haghir H, Sadeghi Y. Modified Fiber Dissection Technique. ASJ 2003; 1 (1) :1-16
URL: http://anatomyjournal.ir/article-1-317-en.html
1- Anatomy Department, Mashhad Medical Sciences University, Mashhad, Iran.
Abstract:   (809 Views)
Introduction: To explain the performance of fiber dissection technique completely together with some modifications to improve the technique based on the authors experience.
Materials & Methods: Fiber dissection technique can be divided into four stages. Stage 1- How to remove the brain from the cranium: the brain should be removed from the cranium less than 12 hours after death. The shorter the interval between the death and removing the brain from the cranium, the better the result would be all efforts must be made in this stage in order to keep the brain intact. To maintain the normal contours of the brain, the basilar artery was ligated and used to suspend the brain in the formalin solution. Stage II- Fixation: the brain must be kept in 4-5 lit. 4% formalin solution to complete the fixation. 4% formalin solution should be changed with new formalin solution after 24 hours and also after 2 weeks. Stage III Freezing: in this stage the brain after washing in water, should be kept in -18 c for one week. If the freezing stage is not successful at the first time, you can repeat it for two or three times. After thawing the freezed brain, it is ready to dissect. Stage IV - Fiber dissection technique: the brain was then dissected using the handmade, thin wooden spatulas with various tip sizes and operating microscope with 6x to 40x magnification.
Results: Fiber dissection technique can demonstrate the internal structure of the brain in three dimensions, increases the contrast between the gray matter and white matter. On the other hand, this technique enables us to follow the fine nerve bundles from their origins to endings. These bundles are separated and dissected as thin round fibers or as white matter sheets.
Conclusion: We suggest using this technique to demonstrate the internal structure of the brain in three dimensions, and to follow the nerve fiber bundles in human brain because of its excellent results and very low expense.
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Type of Study: Original | Subject: Morphometry
Received: 2021/12/25 | Accepted: 2003/02/21 | Published: 2003/02/21

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