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ASJ 2022, 19(1): 39-46 |
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Ehi Oche P, Isaac Dibal N, Chiroma S M, Orendu Oche Attah M. Morphological and Morphometric Variations of Erythrocytes in Clarias Gariepinus, Bufo Bufo, and Agama Agama Using Some Histological Stains. ASJ 2022; 19 (1) :39-46
URL: http://anatomyjournal.ir/article-1-300-en.html
URL: http://anatomyjournal.ir/article-1-300-en.html
1- Department of Human Anatomy, Faculty of Basic Medical Sciences, University of Maiduguri, Nigeria.
Abstract: (1105 Views)
Introduction: Erythrocytes are highly specialized and the most abundant cell type in vertebrates’ blood. Their primary function is the transportation of oxygen to tissues of the body via hemoglobin.
Methods: The study was aimed at evaluating erythrocyte morphology in Clarias gariepinus (C. gariepinus), Bufo bufo (B. bufo), and Agama agama (A. agama) using different stains; Also, morphometric analysis of erythrocytes was evaluated in stains with the best affinity. The blood samples of C. gariepinus, B. bufo, and A. agama were collected using a syringe. Smears from each animal were made on glass slides and stained with Giemsa, Hematoxylin and eosin (H&E), and methylene blue. Erythrocytes diameter was measured using a standardized ocular micrometer. The area and volume of the erythrocytes were calculated.
Results: Erythrocyte’s shapes range from spherical in C. gariepinus to oval in A. agama. Giemsa demonstrated C. gariepinus and B. bufo erythrocytes better than H&E and methylene blue stains, while H&E demonstrated erythrocytes of A. agama better than Giemsa and methylene blue. Erythrocyte’s diameter, area, and volume of B. bufo were significantly higher (P<0.001) when compared with C. gariepinus and A. agama.
Conclusion: Erythrocyte’s shape, size, area, and volume vary in different species and could be used to study evolutionary trends. The variation in erythrocytes size is associated with chromosome number and genome size.
Methods: The study was aimed at evaluating erythrocyte morphology in Clarias gariepinus (C. gariepinus), Bufo bufo (B. bufo), and Agama agama (A. agama) using different stains; Also, morphometric analysis of erythrocytes was evaluated in stains with the best affinity. The blood samples of C. gariepinus, B. bufo, and A. agama were collected using a syringe. Smears from each animal were made on glass slides and stained with Giemsa, Hematoxylin and eosin (H&E), and methylene blue. Erythrocytes diameter was measured using a standardized ocular micrometer. The area and volume of the erythrocytes were calculated.
Results: Erythrocyte’s shapes range from spherical in C. gariepinus to oval in A. agama. Giemsa demonstrated C. gariepinus and B. bufo erythrocytes better than H&E and methylene blue stains, while H&E demonstrated erythrocytes of A. agama better than Giemsa and methylene blue. Erythrocyte’s diameter, area, and volume of B. bufo were significantly higher (P<0.001) when compared with C. gariepinus and A. agama.
Conclusion: Erythrocyte’s shape, size, area, and volume vary in different species and could be used to study evolutionary trends. The variation in erythrocytes size is associated with chromosome number and genome size.
Type of Study: Original |
Subject:
Histology
Received: 2021/11/6 | Accepted: 2022/04/6 | Published: 2022/02/26
Received: 2021/11/6 | Accepted: 2022/04/6 | Published: 2022/02/26
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Anatomical Sciences Journal (ASJ)
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