Volume 14, Issue 1 (Winter 2017 -- 2017)                   ASJ 2017, 14(1): 15-18 | Back to browse issues page

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1- Department of Basic Sciences, Faculty of Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2- PhD Department of Basic Sciences, Faculty of Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3- Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Abstract:   (266 Views)

Introduction: Phasianidae is a non-migratory group of birds belonged to partridges family. One of the well-known species of partridge is Alectoris chukar, which is a medium-sized bird, larger than quails and smaller than pheasants. They are native to Asia, Africa, Europe and the Middle East and belong to the category of gallinaceous birds. Understanding the physiology and immunology of the lymphoid system is incomplete without knowledge of its basic structure that we are going to examine it in these birds. 
Methods: Histomorphological studies were conducted on the spleen of 60 fertilized eggs from the healthy Alectoris chukar birds. Eggs were opened and the spleens of Embryonic Day (ED) 10 to 22 were dissected by dissecting microscope. Gross morphological parameters were studied immediately after collection of samples and then the spleen samples were fixed in Bouin’s solution. After tissue processing, spleens were embedded in paraffin and the prepared sections (5–6 μm) were stained using Hematoxylin and Eosin (H&E) method.
Results: Based on our findings the capsule of spleen was formed by a thin and single layer of the mesothelial cells, on ED 12. At this time, macrophages were observed beneath the endothelium of sinusoids for the first time. The early distinction between the red and white pulp had been obvious on ED 14. The central artery was seen in white pulp of the spleen at ED 20. Reticular cells with large euchromatin nucleus and specified nucleolus were observed in the white pulp. At ED 22, there were Billroth cords in the spleen. Trabecular artery was observed in spleen of the partridge embryos at this time.
Conclusion: This study was the first study on histogenesis and histological of spleen were performed in prenatal partridge. 

Full-Text [PDF 1335 kb]   (123 Downloads)    
Type of Study: Original | Subject: Gross Anatomy
Received: 2016/04/14 | Accepted: 2016/09/10 | Published: 2017/02/1

1. Johnsgard PA. Birds of the great plains: Family phasianidae (quails, pheasants, and partridges). Lincoln: University of Nebraska; 2009.
2. Olah I, Vervelde L. Structure of the avian lymphoid system. Cambridge: Academic Press; 2008. [DOI:10.1016/B978-012370634-8.50005-6]
3. Islam MN, Khan MZ, Jahan MR, Fujinaga R, Yanai A, Kokubu K, et al. Histomorphological study on prenatal development of the lymphoid organs of native chickens of Bangladesh. Pakistan Veterinary Journal. 2012; 32(32):175-8.
4. Seres M, Tirziu E, Nichita I, Cumpanasoiu C, Dobrota M, Hotea I. Development of secondary lymphoid organs in chicken embyos-spleen and gut associated lymphoid tissues. Medicine Veterinary. 2013; 46(2): 118-23.
5. Liman N, Bayram GK. Structure of the quail (Coturnix coturnix japonica) spleen during pre and post-hatching periods. Revue de Médecine Vétérinaire. 2011; 162(1):25-33.
6. Nasu T, Shimizu K, Nakai M. Morphological study of the dove spleen. Poultry Science. 1992; 71(9):1527–30. doi: 10.3382/ps.0711527 [DOI:10.3382/ps.0711527]
7. HashimoTo Y, Sugimura M. Histological and quantitative studies on the postnatal growth of the duck spleen. Japanese Journal of Veterinary Research. 1977; 25(3-4):71-82. PMID: 599756 [PMID]
8. Eurell JA, Frappier BL. Dellmann's textbook of veterinary histology. Hoboken, N. J.: John Wiley & Sons; 2013.
9. Ogata K, Sugimura M, Kudo N. Developmental studies on embryonic and posthatching spleens in chickens with special reference to development of white pulp. Japanese Journal of Veterinary Research. 1977; 25(3-4):83-92. [PMID]
10. Khan MZ, Masum M, Khan MZ, Aziz AR, Nasrin M, Siddique MN. Histomorphology of the lymphoid tissues of broiler chickens in Kelantan, Malaysia (Histomorfologi Tisu Limfa Ayam Pedaging di Kelantan, Malaysia). Sains Malaysiana. 2014; 43(8):1175-9.
11. Akter S, Khan M, Jahan M, Karim M, Islam M. Histomorphological study of the lymphoid tissues of broiler chickens. Bangladesh Journal of Veterinary Medicine. 2008; 4(2):87-92. doi: 10.3329/bjvm.v4i2.1289 [DOI:10.3329/bjvm.v4i2.1289]
12. ] Sultana N, Khan M, Wares M, Masum M. Histomorphological study of the major lymphoid tissues in indigenous ducklings of Bangladesh. Bangladesh Journal of Veterinary Medicine. 2012; 9(1):53-8. doi: 10.3329/bjvm.v9i1.11212 [DOI:10.3329/bjvm.v9i1.11212]