fa Morphometry Morphometry Original Original <strong>Purpose</strong>: The aim of this study was to investigate the effect of buserelin on apoptosis of male germ cells induced by busulfan in adult male mice.&nbsp;<strong>Materials and Methods</strong>: Male adult NMRI mice were divided into four group of eight each. Group 1 (control) administered PBS for 21 days subcutaneously, group 2 given 0.4 mg buserelin for 21 days subcutaneously, group 3 given single dose of 30 mg/kg busulfan intraperitoneally and group 4 given both busulfan and buserelin for 21 days. The animals were sacrificed and their testes were dissected 35 days after the treatment. Evaluations were made by determining Johnson&#39;s score and apoptosis were assayed by terminal- deoxynucleotidyl- transferase-mediated dutp nick end labeling (TUNEL). Statistical analyses were performed using ANOVA test. <strong>Results</strong>: Recovery status and Johnson&#39;s score in group 4were significantly higher than those of busulfan treated group 7.71&plusmn;0.69 VS 4.46&plusmn;0.56 (p< 0.001). Apoptotic cells number cells were significantly more numerous in busulfan treated group than those of control 23.28&plusmn;7.10 VS 3.54&plusmn;1.02 (p<0.001). While buserelin substantially reduced germ cell apoptosis in fourth group 10.50&plusmn;2.91 in comparison with third group 23.28&plusmn;7.10, (p<0.001).<strong>Conclusion:</strong> Administration of buserelin after testicular damage by busulfan enhances the regeneration of spermatogenesis in mouse through inhibition of apoptosis in germ cells. Testis, Spermatogenesis, Apoptosis, Buserelin 0 0 http://anatomyjournal.ir/browse.php?a_code=A-10-304-182&slc_lang=fa&sid=1 Fahimeh Mohammad Ghasemi 10031947532846005963 10031947532846005963 Yes Mohammad Hadi Bahadori 10031947532846005964 10031947532846005964 No Masoumeh Faghani Langeroudi 10031947532846005965 10031947532846005965 No Ebrahim Nasiri 10031947532846005966 10031947532846005966 No Jaffar Soleymanirad 10031947532846005967 10031947532846005967 No